本发明涉及一种藏羚羊-牛种间体细胞核移植胚胎的构建与体外发育方法，该方法以藏羚羊耳朵组织成纤维细胞作为供体细胞，以黄牛、奶牛或牦牛去核卵母细胞作为受体胞质，以无透明带手导式克隆技术构建藏羚羊-牛的种间体细胞核移植胚胎，采用化学辅助融合方法和微穴式胚胎培养系统，获得了高活力的藏羚羊-牛种间体细胞核移植胚胎，为动物克隆技术应用于藏羚羊的保护奠定了基础。 | The invention relates to a construction and ectogenesis method of a Tibetan antelope-cattle interspecific somatic nucleus transferred embryo. In the method, a Tibetan antelope-cattle interspecific somatic nucleus transferred embryo is constructed by using an ear tissue fibroblast of a Tibetan antelope as a donor cell and using an enucleated oocyte of a yellow cattle, a cow or a yak as a recipientcytoplasm by a zona pellucida-free hand guide type clone technology, and a high-activity Tibetan antelope-cattle interspecific somatic nucleus transferred embryo can be obtained by using a chemical auxiliary fusion method and a micro hole type embryo culture system. The method provides the foundation for the application of animal clone technology in the protection of Tibetan antelopes.