本发明涉及一种藏羚羊皮肤成纤维细胞系的构建方法，该方法包括以下步骤：(1)采样；(2)藏羚羊耳部皮肤组织原代培养；(3)传代与纯化；(4)细胞冻存；(5)细胞复苏。本发明通过建立原代培养、细胞传代、细胞冻存与复苏的方法，获得了高活力藏羚羊成纤维细胞系，为深入开展藏羚羊分子生物学及细胞生物学的相关研究提供了研究材料与技术支持。 | The invention relates to a method for constructing a Tibetan antelope skin fibroblast line, which comprises the following steps of: (1) sampling; (2) carrying out primary culture of Tibetan antelope ear skin tissue; (3) carrying out passage and purification; (4) carrying out cell cryopreservation; and (5) carrying out cell recovery. By establishing the method for primary culture, cell passage, cell cryopreservation and cell recovery, the invention obtains a high-activity Tibetan antelope fibroblast line, and provides research materials and technical support for further developing related researches of Tibetan antelope molecular biology and cell biology.