您好，欢迎访问实验动物科技信息规范管理系统
品系名称：B6.FVB-Tg(EIIa-cre)C5379Lmgd/J
基本信息
简称：E2a-Cre;
编号：003724 (Jackson)
科技资源标识：
分类：小鼠,基因工程小鼠,转基因
繁殖方案：Homozygote x Homozygote（♀ × ♂）
代次：？ F5（2015-03-25）
来源：
→ The EIIa-cre plasmid was constructed by replacing the human cytomegalovirus promoter in pBS185 with the adenovirus EIIa promoter from pEII-lacZ. The donating investigator indicates the donor strain was FVB/N and the resulting transgenic mice were bred to C57BL/6NCr (see SNP note below) for 11 generations before arriving at The Jackson Laboratory Repository.（来自Jackson网站）
→ 种鼠来自南京大学；
→ 同胞兄妹交配5代
引种和保存：
2012年10月引入本单位
功能及用途：
This mouse can be used to support research in many areas including:
Research Tools
Cre-lox System
Cre Recombinase Expression
Cre Recombinase Expression: Germline/Embryonic Expression
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Cre related
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System（来自Jackson网站）
生物学特征描述：
This line carries a cre transgene under the control of the adenovirus EIIa promoter that targets expression of Cre recombinase to the early mouse embryo. Cre expression is thought to occur prior to implantation in the uterine wall. A mosaic pattern of expression is commonly observed. Cre-mediated recombination occurs in a wide range of tissues, including the germ cells that transmit the genetic alteration to progeny. These mice may be useful for breeding to other mice carrying loxP-flanked DNA sequences of interest. This would readily generate progeny in which Cre-mediated excision of the targeted sequences has occurred.（来自Jackson网站）
品系名称：B6.C-Tg(CMV-cre)1Cgn/J
基本信息
简称：CMV-cre
编号：006054 (Jackson)
科技资源标识：
分类：小鼠,基因工程小鼠,转基因
繁殖方案：纯合子 x 纯合子（♀ × ♂）
代次：N10F7 F3 F3（2014-11-03）
来源：
→ The cre gene used in this mutant mouse was modified by adding a nuclear localization signal and the human growth hormone gene providing splicing and polyadenylation signals. A construct containing cre coding sequence under the control of a human cytomegalovirus minimal promoter was introduced to BALB/cJ derived BALB/c-I embryonic stem (ES) cells. The resulting mice were backcrossed to the BALB/c background for 8 generations and then backcrossed to the C57BL/6J background for 10 generations.（来自Jackson网站）
→ 2012年9月种鼠从南京大学引进种鼠；
→ 同胞兄妹交配3代；
引种和保存：
2012年9月27日引入本单位
功能及用途：
This mouse can be used to support research in many areas including:
Research Tools
Cre-lox System
Cre Recombinase Expression
Cre Recombinase Expression: Germline/Embryonic Expression
Developmental Biology Research
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
cre related
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System （来自Jackson网站）
生物学特征描述：
In this transgenic strain, deletion of loxP-flanked genes occurs in all tissues, including germ cells. The cre gene in this strain is under the transcriptional control of a human cytomegalovirus minimal promoter and is likely to be expressed before implantation during early embryogenesis. It also appears that the cre gene is X-linked since transgene transmission through males is restricted to female offspring.（来自Jackson网站）
品系名称：FVB/N-Tg(ACTB-cre)2Mrt/J
基本信息
简称：A-cre;Act-Cre;
编号：003376 (Jackson)
科技资源标识：
分类：小鼠,基因工程小鼠,转基因
繁殖方案：Homozygote x Homozygote（♀ × ♂）
代次：？ F6（2015-04-27）
来源：
→ A transgenic insert designed by Drs. Mark Lewandoski and Gail R. Martin (University of California, San Francisco) containing Cre recombinase under the control of the human beta-actin promoter was injected into fertilized FVB/N mouse eggs. Founder line 2 was subsequently established. The mice were maintained on the FVB/N background.（来自Jackson网站）
→ 2013年1月自南京大学引进种鼠；
→ 同胞兄妹交配6代；
引种和保存：
2013年1月19日引入本单位
功能及用途：
This mouse can be used to support research in many areas including:
Research Tools
Cre-lox System
Cre Recombinase Expression
Cre Recombinase Expression: Germline/Embryonic Expression
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Cre related
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System （来自Jackson网站）
生物学特征描述：
This transgenic strain expresses Cre recombinase under the control of the human beta actin gene promoter. Cre recombinase activity is detected in all cells of the embryo by the blastocyst stage of development. It is useful as a "deletor" mouse for virtually any genomic sequence flanked by loxP sites. Homozygotes are viable and fertile.（来自Jackson网站）
品系名称：B6.Cg-Tg(Itgax-cre)1-1Reiz/J
基本信息
简称：Cd11c-Cre;
编号：008068 (Jackson)
科技资源标识：
分类：小鼠,基因工程小鼠,转基因
繁殖方案：sibling x Hemizygote（♀ × ♂）
代次：N12 N3F14 F3 (2015-11-25)
来源：
→ The Itgax-cre (Cd11c-Cre) BAC transgene was designed in the laboratory of Dr. Boris Reizis (Columbia University Medical Center). The mouse bacterial artificial chromosome (BAC) RP24-361C4, containing the integrin alpha X gene (Itgax or Cd11c) but lacking the 5prime end of the adjacentItgam gene, was obtained. A Cre recombinase and a polyA signal sequence was introduced into the first exon of Itgax on the RP24-361C4 BAC via homologous recombination/BAC recombineering. The resulting ~160 kbp transgenic construct was introduced into B6CBAF1/J donor eggs. Founder line 1-1 was consequently established. The mice were crossed to 129S6/SvEvTac for several generations and then backcrossed to C57BL/6 for more than 12 generations prior to sending to The Jackson Laboratory Repository in 2007. Upon arrival, the mice were crossed to C57BL/6J at least once to establish the living colony. （来自Jackson网站）
→ 2013年6月从南京大学引进种鼠；
→ 同胞兄妹交配3代
引种和保存：
2013年6月18日引入本单位
功能及用途：
Research Applications
This mouse can be used to support research in many areas including:
Developmental Biology Research
Internal/Organ Defects
hematopoietic defects
Lymphoid Tissue Defects
hematopoietic defects
Hematological Research
Hematopoietic Defects
Immunology, Inflammation and Autoimmunity Research
Lymphoid Tissue Defects
hematopoietic development
Research Tools
Cre-lox System
Cre Recombinase Expression
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Tissue/Cell Markers: Cre-lox System
Immunology, Inflammation and Autoimmunity Research
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System（来自Jackson网站）
生物学特征描述：
Mice hemizygous for the Itgax-cre (Cd11c-Cre) transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These Cd11c-Cre transgenic mice express Cre recombinase under the control of the mouse integrin alpha X (Cd11c) promoter. Cre-mediated recombination is detected in more than 95% of conventional CD11chigh dendritic cells both from lymphoid organs and from non-lymphoid tissues such as lung and epidermis, and in 50-80% of plasmacytoid dendritic cells. The dendritic cell compartment of transgenic mice is normal. Relatively low amounts of recombination are detected in lymphocytes (<10%), NK cells (12%), and myeloid cells (<1%). No increase of recombination frequency was observed in CD11clow- activated T cells. These Cd11c-cre transgenic mice (as well as CD11c-Cre-GFP transgenic mice (see Stock No.007567)) are an effective tool for generating tissue-specific targeted mutants for studying dendritic cell homeostasis and function.（来自Jackson网站）
品系名称：NOD/ShiLt-Tg(Foxp3-EGFP/cre)1cJbs/J
基本信息
简称：NOD.Foxp3-cre;
编号：008694 (Jackson)
科技资源标识：
分类：小鼠,基因工程小鼠,转基因
繁殖方案：Noncarrier x Hemizygote（♀ × ♂）
代次： N1F8 F5 （2015-4-20）
来源：
→ The 188 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-143D8, containing the intact Foxp3 gene was modified by inserting an enhanced green fluorescent protein (EGFP) fused to a codon optimized "humanized" cre recombinase. This BAC transgene was injected into NOD/ShiLtJ fertilized oocytes and maintained on the same background. In 2008, the T1DR received this NOD coisogenic strain.（来自Jackson网站）
→ 2013年1月从南京大学引进种鼠；
→ 同胞兄妹交配5代
引种和保存：
2012年1月19日引入本单位
功能及用途：
This mouse can be used to support research in many areas including:
Diabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains
NOD Transgenics
Research Tools
Cre-lox System
Cre Recombinase Expression
Fluorescent Proteins
Immunology, Inflammation and Autoimmunity Research
T cell specific surface marker
GFP related
Research Tools
Fluorescent Proteins
Cre related
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System（来自Jackson网站）
生物学特征描述：
Mice hemizygous for the Foxp3-EGFP/cre BAC transgene are viable and fertile. They express an enhanced green fluorescent protein and codon optimized "humanized" cre under the control of the mouse Foxp3 promoter. Transgene expression is specific to Cd4 Cd25 highCd127 low T cells from the lymph nodes, spleen and thymus. GFP expression is restricted to FoxP3 cells. This mutant mouse strain may be useful for fluorescently labeling Foxp3 T-cells to study regulatory T-cell function in autoimmunity specifically, type 1 diabetes. （来自Jackson网站）
品系名称：B6.129S1-Tlr1tm1Flv/J
基本信息
简称：TLR1-;
编号：007020 (Jackson)
科技资源标识：
分类：小鼠,基因工程小鼠,靶突变KO
繁殖方案：Homozygote x Homozygote（♀ × ♂）
代次：N10 ? F6（2015-04-27）
来源：
→ A targeting vector incorporating a loxP-flanked neomycin cassette in reverse orientation (derived from plasmid pMC1neo poly A, Stratagene) was used to replace the coding exon. The construct was introduced to 129S1/Sv-derived W9.5 embryonic stem (ES) cells. This resultant line has been backcrossed to C57BL/6 by the donating laboratory for ten generations.（来自Jackson网站）
→ 种鼠来源于南京大学；
→ 同胞兄妹交配6代；
引种和保存：
2011年9月16日引入本单位
功能及用途：
This mouse can be used to support research in many areas including:
Immunology, Inflammation and Autoimmunity Research
Growth Factors/Receptors/Cytokines
Immunodeficiency
Vaccine Development （来自Jackson网站）
生物学特征描述：
Homozygotes are viable and fertile. IL6 production is significantly impaired in homozygous macrophages treated with Borrelia burgdorferi's OspA (outer-surface lipoprotein). No expression products from the gene are found in either lipopolysacharide (LPS)-treated or untreated bone marrow-derived macrophages, as assayed by RT-PCR. This strain may be useful in vaccine development.（来自Jackson网站）
品系名称：B6;SJL-Tg(Thy1-TARDBP)4Singh/J
基本信息
简称：TAR4;
编号：012836 (Jackson)
科技资源标识：
分类：小鼠,基因工程小鼠,转基因
繁殖方案：sibling x Hemizygote（♀ × ♂）
代次：？ N6
来源：
→ Mice hemizygous for this TDP43 transgene contain the mouse Thy1 promoter driving expression of the human TAR DNA binding protein (TARDBP or TDP-43) gene in neurons throughout the central nervous system. These mice may be useful in studying the accumulation of TDP-43 in neurodegenerative diseases such as ALS and FTLD.（来自Jackson网站）
→ 2012年8月24从南京大学引进种鼠；
→ 回交6代
引种和保存：
2012年8月24日从南京大学引入本单位
功能及用途：
This mouse can be used to support research in many areas including:
Neurobiology Research
Amyotrophic Lateral Sclerosis (ALS)
Ataxia (Movement) Defects
Neurodegeneration（来自Jackson网站）
生物学特征描述：
Mice hemizygous for this TDP43 transgene are viable, fertile, and express human TAR DNA binding protein (TARDBP or TDP-43) gene. Expression is directed in neurons throughout the central nervous system by the mouse Thy1 promoter. These TDP-43 transgenic mice exhibit a dose-dependent degeneration of cortical and spinal motor neurons and develop a spastic quadriplegia reminiscent of Amyotrophic Lateral Sclerosis (ALS). They also show degeneration of nonmotor cortical and subcortical neurons characteristic of frontotemporal lobar degeneration (FTLD).Neurons in the affected spinal cord and brain regions showed accumulation of TDP-43 nuclear and cytoplasmic aggregates that were both ubiquitinated and phosphorylated as observed in human ALS/FTLD disease. These mice show abnormal limb reflex by 14 months and 40% reduced motor performance by 15 months of age. The Donating Investigator maintains these mice as hemizygotes and mates carriers to produce homozygotes, which exhibit an accelerated phenotype. Homozygotes develop an abnormal hindlimb clasping reflex by 14 days, and a 2-fold decrease in the stride of hindlimbs and forelimbs. Their footprints were characterized by separated forelimb and hindlimb prints, markedly wide-based stance, small stride, and frequent off-line stumbling. In addition, TDP-43 was present in the nucleus and cytoplasm of 10% of spinal neurons in homozygotes and less than 1% in hemizygotes. These mice may be useful in studying the accumulation of TDP-43 in neurodegenerative diseases such as ALS and FTLD.（来自Jackson网站）
品系名称：B6.Cg-Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas/Mmjax
基本信息
简称：5XFAD;
编号：008730 (Jackson)
科技资源标识：
分类：小鼠,基因工程小鼠,转基因
繁殖方案：Noncarrier × Hemizygote（♀ × ♂）
代次：? F7（2014-11-2
来源：
→ A transgene was designed with a mutant human amyloid beta (A4) precursor protein (APP) cDNA sequence (altered to include the APP K670N/M671L (Swedish) I716V (Florida) V717I (London) Familial Alzheimer's Disease (FAD) mutations) inserted into exon 2 of the mouse Thy1 gene. A second transgene was designed with a mutant human presenilin 1 (Alzheimer disease 3) (PSEN1 or PS1) cDNA sequence (altered to include the PS1 M146L L286V FAD mutations) inserted into exon 2 of the mouse Thy1 gene. Both transgenes were added together in equal proportions and co-injected into the pronuclei of single-cell "C57/B6XSJL" hybrid embryos. Founders from the highest APP expressing line (Tg6799) were bred with (B6/SJL)F1 for more than 10 generations with stable germ-line transmission and expression of both transgenes, demonstrating that these "5XFAD" mice breed as single transgenics. The mice were then backcrossed to C57BL/6J mice using a speed congenic protocol and the retinal degeneration allele Pde6brd1 was bred out of the strain. Of note, the APP transgene includes the 5' untranslated region and thus contains a putative interleukin-1beta translational enhancer element.（来自Jackson网站）
→ 种鼠来自首都医科大学委托保种，扩繁；
→ 同胞兄妹交配7代
引种和保存：
2011年？月引入本单位
功能及用途：
This mouse can be used to support research in many areas including:
Neurobiology Research
Alzheimer's Disease
APP and PSEN1 mutants
Presenilin mutants
strains expressing mutant APP
Neurodegeneration
Research Tools
Neurobiology Research
生物学特征描述：
Hemizygous mice are viable and fertile. These 5XFAD transgenic mice overexpress both mutant human APP(695) with the Swedish (K670N, M671L), Florida (I716V), and London (V717I) Familial Alzheimer's Disease (FAD) mutations and human PS1 harboring two FAD mutations, M146L and L286V. Expression of both transgenes is regulated by neural-specific elements of the mouse Thy1 promoter to drive overexpression in the brain. Mice from this founder line have high APP expression correlating with high burden and accelerated accumulation of the 42 amino acid species of beta-amyloid (Aβ-42). 5XFAD mice generate Aβ-42 almost exclusively and rapidly accumulate massive cerebral levels. On the B6SJL F1 genetic background (see MMRRC stock 34840), intraneuronal Abeta;-42 accumulation is observed starting at 1.5 months of age, just prior to amyloid deposition and gliosis, which begins at two months of age. On a congenic C57BL/6J genetic background (see MMRRC stock 34848) it has been the observation of the MMRRC that this phenotype is not as robust as that demonstrated in the B6SJL hybrid background (view data). In addition, these mice have reduced synaptic marker protein levels, increased p25 levels, neuron loss, and memory impairment in the Y-maze test. 5XFAD transgenic mice rapidly recapitulate major features of Alzheimer's Disease amyloid pathology and may be useful models of intraneuronal Aβ-42 induced neurodegeneration and amyloid plaque formation.
（来自Jackson网站）
品系名称：B6.129P2-Apoetm1Unc/J
基本信息
简称：ApoE KO
编号：002052 (Jackson)
科技资源标识：
分类：小鼠,基因工程小鼠,靶突变KO
繁殖方案：Homozygote x Homozygote（♀ × ♂）
代次：？ F10 F6（2015-03-25）
来源：
→ The Apoetm1Unc mutant strain was developed in the laboratory of Dr. Nobuyo Maeda at The University of North Carolina at Chapel Hill. The 129P2/OlaHsd-derived E14Tg2a ES cell line was used. The plasmid used is designated as pNMC109 and the founder line is T-89 in the primary reference. The C57BL/6J strain was produced by backcrossing the Apoetm1Unc mutation 10 times to C57BL/6J mice. Previously mice backcrossed 6 times (N6) to C57BL/6J mice were distributed solely. Mice from the N6 generation are homozygous for pink-eyed dilution p giving them pink eyes and a silver coat color. The E14Tg2a ES cell line carries this recessive mutation which remains linked to the targeted Apoe gene on Chromosome 7 at this backcross generation. Mice from the N6 colony are no longer available for distribution.（来自Jackson网站）
→ 种鼠来自南京大学；
→ 同胞兄妹交配6代
引种和保存：
2012年4月引入本单位
功能及用途：
This mouse can be used to support research in many areas including:
Cardiovascular Research
Atherosclerosis
Diet-Induced Atherosclerosis
Heart Abnormalities
Hypercholesterolemia
Hypertriglyceridemia
Other
altered fat metabolism
altered lipoprotein profile
Vascular Defects
Diabetes and Obesity Research
Obesity Without Diabetes
diet-induced
Metabolism Research
Lipid Metabolism
Neurobiology Research
Alzheimer's Disease
APOE mutants
Behavioral and Learning Defects
Neurodegeneration
Research Tools
Cardiovascular Research
Metabolism Research
Neurobiology Research
Apoetm1Unc related
Cardiovascular Research
Atherosclerosis
Hypercholesterolemia（来自Jackson网站）
生物学特征描述：
Mice homozygous for the Apoetm1Unc mutation show a marked increase in total plasma cholesterol levels that are unaffected by age or sex. Fatty streaks in the proximal aorta are found at 3 months of age. The lesions increase with age and progress to lesions with less lipid but more elongated cells, typical of a more advanced stage of pre-atherosclerotic lesion. Moderately increased triglyceride levels have been reported in mice with this mutation on a mixed C57BL/6 x 129 genetic background. Aged apoE-deficient mice (>17 months) have been shown to develop xanthomatous lesions in the brain consisting mostly of crystalline cholesterol clefts, lipid globules, and foam cells. Smaller xanthomas were seen in the choroid plexus and ventral fornix. Additional studies indicate that apoE-deficient mice have altered responses to stress, impaired spatial learning and memory, altered long term potentiation, and synaptic damage.（来自Jackson网站）
品系名称：129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J
基本信息
简称：FLP;
编号：003946 (Jackson)
科技资源标识：
分类：小鼠,基因工程小鼠,靶突变KO
繁殖方案：Homozygote x Homozygote（♀ × ♂）
代次：？ F9 F6 （2015-03-25）
来源：
→ A knock-in construct (R26Fki) was built by inserting a FLPe cassette into a ROSA26 knock-in vector. This construct includes a neomycin resistance gene driven by the mouse phosphoglycerate kinase promoter (PGK neo) and polyadenylation signal sequence. The construct was electroporated into 129S4/S4JaeSor-derived AK7 embryonic stem (ES) cells, thereby disrupting the Gt(ROSA)26Sor locus. Correctly targeted ES cells were utilized to generate chimeras. This strain originated and is maintained on a 129S4/SvJaeSor background.（来自Jackson网站）
→ 种鼠来自南京大学；
→ 同胞兄妹交配6代；
引种和保存：
2012年4月13日引入本单位
功能及用途：
This mouse can be used to support research in many areas including:
Research Tools
FLP-FRT System
FLP Recombinase Expression
FLP Recombinase Expression: Germline/Embryonic Expression
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: FLP recombinase
FLP related
Research Tools
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: FLP recombinase（来自Jackson网站）
生物学特征描述：
Homozygous FLPeR mice are viable and fertile, with widespread expression of the FLPe variant of the Saccharomyces cerevisiae FLP1 recombinase gene driven by the Gt(ROSA)26Sor promoter. The FLPe recombinase variant exhibits enhanced thermostability with recombination activity being four-fold and ten-fold that of wild type FLP at 37C and 40C, respectively. The Gt(ROSA)26Sor promoter drives expression from preimplantation onward and extensive target gene recombination can be achieved in most tissue types, including cells of the developing germ line. The knock-in construct also includes a neomycin resistance gene driven by the mouse phosphoglycerate kinase promoter (PGK neo) and polyadenylation signal sequence. When bred with mice containing a frt-flanked sequence of interest, FLP-mediated recombination will result in deletion of the frt-flanked sequence(s) in the offspring. This FLP deleter strain may be useful for generating frt-FLP conditional mutations and serves as a source of FLP1-expressing primary cell lines.（来自Jackson网站）
实验动物科技信息规范管理系统